Journal: Journal of Agricultural and Food Chemistry
Article Title: Functionalization of Amorphous and Crystalline Calcium Phosphate Nanoparticles with Urea for Phosphorus and Nitrogen Fertilizer Applications
doi: 10.1021/acs.jafc.5c03970
Figure Lengend Snippet: (a) Microbial community structure at the family level and (b) microbial community structure at the genus level for ACP, ACP-U, and Control (MCP) treatments. Stacked bar plots showing the relative abundance of microbial communities at the (a) family and (b) genus levels across different treatments. Each treatment includes six columns representing biological replicates, with each column corresponding to one sample. Relative abundances were calculated based on 16S rRNA gene sequencing data and normalized within each sample. The plots display the top 20 most abundant families and genera, respectively, with all remaining taxa grouped under “Others.” Each panel also includes a Venn diagram showing the number and percentage of families (a) and genera (b) that are unique to or shared among treatments. All plots were generated in R (version 4.4.3) using the “ggplot2” and “VennDiagram” packages.
Article Snippet: PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK-RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set and Platinum SuperFi II DNA polymerase (Invitrogen).
Techniques: Control, Sequencing, Generated